Understanding Purity: How HPLC and Mass Spectrometry Verify Our 98%+ Guarantee.

In the world of professional laboratory research, a guarantee is only as good as the data that supports it. When a researcher purchases peptides for sensitive assays, a purity claim of “98%+” needs to be backed by verifiable, scientific evidence.
At Peptide24h, we don’t just state our purity levels; we prove them through rigorous analytical testing using industry-standard methodologies: High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS).
These two techniques work in tandem to confirm that what arrives in your lab vial is exactly what you ordered a high-quality, research-grade peptide essential for valid experimental outcomes.

1. The Gold Standard: High-Performance Liquid Chromatography (HPLC).

HPLC is the primary method used to quantify purity and separate a compound from its impurities. It is the gold standard in the peptide industry.
How it Works:
A dissolved peptide sample is injected into a specialized column packed with microscopic particles. A liquid solvent (the mobile phase) is pumped through the column under high pressure. As the mixture travels through the column, different components of the sample (the primary peptide and impurities) interact differently with the packing material.
Purer components travel through the column faster or slower depending on their properties.
A detector at the end of the column measures the amount of substance exiting over time.
The result is a chromatogram: a graph with peaks. The larger the main peak relative to all other peaks, the purer the sample.
Our Guarantee: The resulting chromatogram on your Certificate of Analysis (CoA) visually confirms that the primary peptide accounts for 98% or more of the total area detected, backing our guarantee with hard data.

2. The Identity Check: Mass Spectrometry (MS).

Purity is only half the story. You need to know if your sample is the correct peptide sequence. This is where Mass Spectrometry (MS) becomes indispensable.
How it Works:
MS analysis measures the mass-to-charge ratio of the ions in a sample. It effectively determines the precise molecular weight of the compound.
Our Guarantee: The theoretical molecular weight of the peptide sequence you ordered is calculated beforehand. The MS analysis confirms the exact measured weight of the synthesized product. A match between the theoretical and measured weights is the definitive proof that the structure of the peptide is correct.
The Power of Both: Verification & Validation
Using HPLC and MS together provides a robust two-step verification process:
HPLC confirms how much of the sample is the desired product (purity).
MS confirms what the product is (identity).
At Peptide24h, this dual-verification process is applied to every batch. Our commitment to using independent third-party labs for these analyses ensures unbiased results and complete transparency.
When you see our 98%+ guarantee, you are seeing a commitment to quality control backed by rigorous scientific methodology. We provide you with the data you need so you can proceed with confidence in your research.

DISCLAIMER: All products provided by Peptide24h are strictly for in-vitro laboratory research and development purposes only. They are not intended for human or animal consumption, diagnostic use, or therapeutic procedures. Customers must assume all responsibility for compliance with local regulations. We cannot provide medical advice.